Data formats

• Barcode list format (tab- or space-separated table)

  Fields cannot contain spaces

  Required fields (in order):

  1. Barcode name - Unique identifier for the barcode
  2. Barcode type - Type of barcode (e.g., i5, i7)
  3. Barcode sequence in physical oligo - The actual barcode sequence
  4. Physical primer/adapter backbone sequence - Backbone sequence where N characters indicate barcode position
  5. Orientation of physical barcode in final library - Either "forward" or "reverse"

  Example:

  Barcode name	Barcode type	Barcode sequence in physical oligo	Physical primer/adapter backbone sequence (N characters indicate barcode position)	Orientation of physical barcode in final library
  P7_A01	i7	CGGTTCAA	aaaaaaaaaaaaaaannnnnnnngtgtgtgtgtgtgtgtgtgtgt	reverse
  P7_A02	i7	GCTGGATT	aaaaaaaaaaaaaaannnnnnnngtgtgtgtgtgtgtgtgtgtgt	reverse
  P7_A03	i7	TAACTCGG	aaaaaaaaaaaaaaannnnnnnngtgtgtgtgtgtgtgtgtgtgt	reverse
  
  

  Plate Coordinates: If the barcode name ends with a plate coordinate pattern (e.g., _A01, -B12), the coordinate will be automatically extracted and stored. The pattern is: [_-][A-H]\d{1,2} where the letter represents the row (A-H) and the number represents the column (1-12). Examples: P7_A01, P7_A02, P7_B12, P5-H8